mouse anti p p38 antibody  (Santa Cruz Biotechnology)

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet: ( A ) Isolated ISCs cultured using a medium supplemented with or without 1 μM NIC combined with 5 nM Sotrastaurin (PKC inhibitor) (3 wells/group). ( B ) Immunoblotting analyses of p38, p-p38, p-S6, or S6 in ISCs isolated from mice treated with vehicle or NIC. ( C ) Isolated ISCs were cultured in a medium with or without 1 μM NIC and 1 μM AKT inhibitor VIII (3 wells/group). ( D ) Isolated ISCs were cultured in a medium with or without 1 μM Nicotine and 5 μM SB 203580 (3 wells/group). ( E ) Isolated ISCs were cultured in a medium with or without 1 μM NIC and 1 μM Rapamycin (3 wells/group). Significant differences are denoted by p values (Student’s t-test). See also . Figure 4—figure supplement 1—source data 1. The quantification of colonies in organoid assay. Figure 4—figure supplement 1—source data 2. Immunoblotting data with labeling. Figure 4—figure supplement 1—source data 3. Immunoblotting raw data.

Article Snippet: The following antibodies, obtained from different sources, were used for immunoblotting: mouse anti-β-actin (sc-47778; Santa Cruz), mouse anti-YAP (Santa Cruz sc-101199), mouse anti-TAZ (Santa Cruz sc-293183), mouse anti-α7-AchR (Santa Cruz sc-58607), mouse anti-Notch1 (Santa Cruz sc-376403), mouse anti-Jagged1 (Santa Cruz sc-390177), mouse anti-Jagged2 (Santa Cruz sc-515725), mouse anti-Hes5 (Santa Cruz sc-293445), mouse anti-p38 (Santa Cruz sc-81621), mouse anti-phospho-p38 (Santa Cruz sc-166182), rabbit anti-S6 (Cell Signaling 2217), and rabbit anti-phospho-S6 Ser235/236 (Cell Signaling 4858).

Techniques: Isolation, Cell Culture, Western Blot, Labeling

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: The following antibodies, obtained from different sources, were used for immunoblotting: mouse anti-β-actin (sc-47778; Santa Cruz), mouse anti-YAP (Santa Cruz sc-101199), mouse anti-TAZ (Santa Cruz sc-293183), mouse anti-α7-AchR (Santa Cruz sc-58607), mouse anti-Notch1 (Santa Cruz sc-376403), mouse anti-Jagged1 (Santa Cruz sc-390177), mouse anti-Jagged2 (Santa Cruz sc-515725), mouse anti-Hes5 (Santa Cruz sc-293445), mouse anti-p38 (Santa Cruz sc-81621), mouse anti-phospho-p38 (Santa Cruz sc-166182), rabbit anti-S6 (Cell Signaling 2217), and rabbit anti-phospho-S6 Ser235/236 (Cell Signaling 4858).

Techniques:

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: Antibody , mouse monoclonal anti-phospho-p38 , Santa Cruz , #sc-166182 , WB (1:100).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: A supercritical oil extract of Schisandra chinensis seeds ameliorates Huntington’s disease-like symptoms and neuropathology: the potential role of anti-oxidant and anti-inflammatory effects

doi: 10.3389/fphar.2024.1471024

Figure Lengend Snippet: SOSC inhibits inflammatory signaling pathways (MAPKs and NF-κB) in the striatum after 3-NPA intoxication. (A–F) Twelve hours after the final (4 th ) 3-NPA intoxication, striatal lysates (n = 5 per group) from Sham, 3-NPA, 3-NPA + SOSC (300 mg/kg/day), and SOSC (300 mg/kg/day) groups were subjected to Western blot assay (A) and quantified (B–F) . SOSC decreased protein expression levels of p-ERK (A, B) , p-JNK (A, C) , p-p38 (A, D) , p-IκB (A and E), and p-NF-κB (A, F) . Data are expressed as mean ± SEM (one-way ANOVA with post hoc test; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. Sham group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. 3-NPA group). 3-NPA, 3-nitropropionic acid; ERK, extracellular signal-regulated kinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IκB, inhibitor of NF-κB; JNK, Jun amino-terminal kinases; NF-κB, nuclear factor kappa B; SOSC, seed oil of Schisandra chinensis.

Article Snippet: Rabbit anti-extracellular signal-regulated kinases 1/2 (ERK1/2; #9102), rabbit anti-interleukin-1 beta (IL-1β; #31202), rabbit anti-IL-6 (#12912), rabbit anti-phospho (p)-ERK1/2 (#9101), rabbit anti-c-Jun N-terminal kinases (JNK; #9258), rabbit anti-p-JNK (#4668), rabbit anti-NF-kappa-B inhibitor alpha (IκB-α; #4814), rabbit anti-p-IκB-α (#2859), rabbit anti-pro-caspase-3 (#9662), rabbit anti-cleaved caspase-3 (#9661), rabbit anti-p38 mitogen-activated protein kinase (MAPK; #9212), mouse anti-p-p38 MAPK (#9216), and rabbit anti-transforming growth factor-beta (TGF-β; #3711) antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Protein-Protein interactions, Western Blot, Expressing

Journal: Oncology letters

Article Title: Co‑expression of SLC20A1 and ALDH1A3 is associated with poor prognosis, and SLC20A1 is required for the survival of ALDH1‑positive pancreatic cancer stem cells.

doi: 10.3892/ol.2024.14558

Figure Lengend Snippet: Figure 4. Western blot analysis of proteins associated with p38, JNK, p44/42 and Akt signaling following SLC20A1 knockdown in PANC‑1 and MIA‑PaCa‑2 cells. SLC20A1 was knocked down in PANC‑1 and MIA‑PaCa‑2 cells by incubation with SLC20A1 siRNA (or NC siRNA) in a two‑dimensional monolayer culture for 48 h, and the subsequent three‑dimensional tumorspheres were cultured for 72 h (PANC‑1) or 24 h (MIA‑PaCa‑2). (A) Representative western blot images. Relative SLC20A1 mRNA expression following transfection with SLC20A1 siRNA or NC siRNA in (B) PANC‑1 and (C) MIA‑PACA‑2 cells. Levels of p‑p38 in (D) PANC‑1 and (E) MIA‑PaCa‑2 cells. Levels of p‑p44/42 in (F) PANC‑1 and (G) MIA‑PaCa‑2 cells. Level of p‑JNK in (H) PANC‑1 and (I) MIA‑PaCa‑2 cells. Level of p‑AKT S473 in (J) PANC‑1 and (K) MIA‑PaCa‑2 cells. The molecular weight of phosphorylated proteins and proteins is shown on the right of the blot images. (D‑K) Evaluation of the ratio of phosphorylated protein/total protein. *P<0.05, **P<0.01 vs. NC; unpaired Student's t‑test. SLC20A1, solute carrier family 20 member 1; siRNA, small interfering RNA; NC, negative control; KD, knockdown; p‑, phosphorylated.

Article Snippet: The primary antibodies were as follows: mouse anti‐p‐p38 monoclonal antibody (mAb) (cat. no. 9216s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p38 polyclonal antibody (pAb) (cat. no. 9212s; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:3,000); rabbit anti‐phosphorylated (p)‐c‐Jun N‐terminal Kinase (JNK) pAb (cat. no. 9251s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐JNK pAb (cat. no. 9252s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p‐p44/p42 pAb (cat. no. 9101s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p44/p42 pAb (cat. no. 9102s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐p‐Akt S473 mAb (cat. no. 4060s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐Akt mAb (cat. no. 2938s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti‐ALDH1A1 mAb (cat. no. ab52492; Abcam; 1:5,000); rabbit anti‐ALDH1A3 pAb (cat. no. PA5‐29188; Thermo Fisher Scientific, Inc.; 1:5,000); and mouse anti‐β‐actin mAb (cat. no. 60008‐1‐Ig; ProteinTech Group, Inc.; 1:10,000).

Techniques: Western Blot, Knockdown, Incubation, Cell Culture, Expressing, Transfection, Molecular Weight, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: Co‑expression of SLC20A1 and ALDH1A3 is associated with poor prognosis, and SLC20A1 is required for the survival of ALDH1‑positive pancreatic cancer stem cells

doi: 10.3892/ol.2024.14558

Figure Lengend Snippet: Western blot analysis of proteins associated with p38, JNK, p44/42 and Akt signaling following SLC20A1 knockdown in PANC-1 and MIA-PaCa-2 cells. SLC20A1 was knocked down in PANC-1 and MIA-PaCa-2 cells by incubation with SLC20A1 siRNA (or NC siRNA) in a two-dimensional monolayer culture for 48 h, and the subsequent three-dimensional tumorspheres were cultured for 72 h (PANC-1) or 24 h (MIA-PaCa-2). (A) Representative western blot images. Relative SLC20A1 mRNA expression following transfection with SLC20A1 siRNA or NC siRNA in (B) PANC-1 and (C) MIA-PACA-2 cells. Levels of p-p38 in (D) PANC-1 and (E) MIA-PaCa-2 cells. Levels of p-p44/42 in (F) PANC-1 and (G) MIA-PaCa-2 cells. Level of p-JNK in (H) PANC-1 and (I) MIA-PaCa-2 cells. Level of p-AKT S473 in (J) PANC-1 and (K) MIA-PaCa-2 cells. The molecular weight of phosphorylated proteins and proteins is shown on the right of the blot images. (D-K) Evaluation of the ratio of phosphorylated protein/total protein. *P<0.05, **P<0.01 vs. NC; unpaired Student's t-test. SLC20A1 , solute carrier family 20 member 1; siRNA, small interfering RNA; NC, negative control; KD, knockdown; p-, phosphorylated.

Article Snippet: The primary antibodies were as follows: mouse anti-p-p38 monoclonal antibody (mAb) (cat. no. 9216s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p38 polyclonal antibody (pAb) (cat. no. 9212s; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:3,000); rabbit anti-phosphorylated (p)-c-Jun N-terminal Kinase (JNK) pAb (cat. no. 9251s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-JNK pAb (cat. no. 9252s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p-p44/p42 pAb (cat. no. 9101s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p44/p42 pAb (cat. no. 9102s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-p-Akt S473 mAb (cat. no. 4060s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-Akt mAb (cat. no. 2938s; Cell Signaling Technology, Inc.; 1:3,000); rabbit anti-ALDH1A1 mAb (cat. no. ab52492; Abcam; 1:5,000); rabbit anti-ALDH1A3 pAb (cat. no. PA5-29188; Thermo Fisher Scientific, Inc.; 1:5,000); and mouse anti-β-actin mAb (cat. no. 60008-1-Ig; ProteinTech Group, Inc.; 1:10,000).

Techniques: Western Blot, Knockdown, Incubation, Cell Culture, Expressing, Transfection, Molecular Weight, Small Interfering RNA, Negative Control